IDENTIFICATION OF Escherichia coli AND Salmonella sp. ON SOME PLANTATION PRODUCTS
Main Article Content
Christina Oktora Matondang
Muklasin
Nurhayati
Identification of Escherichia coli and Salmonella sp. In Several Plantation Products, the aim is to explain the results of the identification test of E. coli and Salmonella sp. on plantation products. The research was carried out with 4 treatments and 2 replications. Each replication consisted of 5 dilutions. The treatments used were cocoa powder, compost, water and lettuce in selective growing media (EMBA and SSA). The parameters observed were to see whether there were E. coli or Salmonella sp. that grew in the four samples, the number of colonies and color changes that occurred as well as gas or bubbles produced in the fermentation test of sugars (glucose, sucrose and lactose). Based on the test results, it was found that E. coli and Salmonella were not present in the cocoa powder samples in EMBA and SSA media. In the compost sample, the bacteria E. coli grew on EMBA media, but Salmonella bacteria did not grow on SSA media. Lettuce samples showed E. coli growing on EMBA media, while on SSA media there was E. coli and Salmonella bacteria. Meanwhile, in water samples, E. coli, Salmonella and other bacteria grew on EMBA media. And on SSA media, Salmonella and Shigella bacteria grow. In the fermentation test of sugars such as glucose, sucrose and lactose, the media changes color to yellow and there are gas/air bubbles. This shows that bacteria can ferment carbohydrates and can produce gas. Salmonella and other bacteria grow on EMBA media. And on SSA media, Salmonella and Shigella bacteria grow. In the fermentation test of sugars such as glucose, sucrose and lactose, the media changes color to yellow and there is gas/air bubbles. This shows that bacteria can ferment carbohydrates and can produce gas. Salmonella and other bacteria grow on EMBA media. And on SSA media, Salmonella and Shigella bacteria grow. In the fermentation test of sugars such as glucose, sucrose and lactose, the media changes color to yellow and there is gas/air bubbles. This shows that bacteria can ferment carbohydrates and can produce gas.
Achmad S.A., 1986. Kimia Organik Bahan Alam. Penerbit Karunika, Jakarta.
Anonimus. 1992. Cara Uji Cemaran Mikroba, Standar Nasional Indonesia, Dewan Standarisasi Nasional.
Anonimus. 2004. Buku Ajar Mikrobiologi. Tim Mikrobiologi FKH Unsyiah. Banda Aceh.
Anonimus, 2007. Jahe, Penghangat Badan dan Obat Pencernaan. http://www.apoteker.info/Pojok%20Herbal/PH-pegagan.htm.
Anonimus. 2008. Salmonella sp. http://www.food-info.net/id/bact/salm.htm.
Brooks G.F., J.S. Butel, L.N. Ornston, 1996. Jawetz Melnick Adelberg, Medical Microbiology. Edisi ke-20. Alih bahasa oleh Nugroho E dan Maulany RF. EGC Penerbit Buku Kedokteran, Jakarta.
Buckle, K.A., R.A. Edward, G.H. Fleet dan M. Woton 1985. Ilmu Pangan, Universitas Indonesia. Press, Jakarta.
Budiarti, T. 2007. Fisiologi Mikroba. Bumi Aksara. Jakarta
Chang H.M. and But, P.P.H; 1987, Pharmacology and Application of Chinese Materia Medica Vol. I The Chinese Medicinal Material Research Centre, The Chinese University of Hongkong.
Dzulkarnain B., D. Sundari, A. Chozin, 1996. Tanaman Obat Bersifat Antibakteri di Indonesia. Cermin Dunia Kedokteran, 110:35-48.
Hadioetomo, R. S., 1993. Mikrobiologi Dasar Dalam Praktek. Teknik dan Prosedur Dasar Laboratorium. Laboratorium Mikrobiologi FMIPA. IPB. Bogor.
Hartoko, 2008. Mikroba
Patogen. http://hartoko.wordpress.com/keamanan-pangan/mikroba- patogen. diakses pada tanggal 03 Mei 2009.
Jain, S.K., dan S. Lata, 1996, Unique Indigenous Amazonian Uses of Some Plants Growing in India, IK Monitor 4(3) article. http://www.nuffic.nl/ciran/ikdm. Accesed 2000 December 5.
Jawetz, E., 1995, Mikrobiologi untuk Profesi Kesehatan, Penerbit Buku Kedokteran EGC, Jakarta.
Jawetz, E., J. L. Melnick dan E. A. Adelberg, 1996. Mikrobiologi Kedokteran. Edisi 20. Penerbit Buku Kedokteran. EGM. Jakarta.
Koswara, S., 1995. Jahe dan Hasil Olahannya, Pustaka Sinar Harapan, Jakarta.
Krieg, N.R. and J.G. Holt. 1984. Bergey’s Manual of Determinative Bacteriology. MacMillan, Baltimore.
Masduki I., 1996. Efek Antibakteri Ekstrak Biji Pinang (Areca catechu) terhadap S. aureus dan E. coli. Cermin Dunia Kedokteran 109 : 21- 24.
Michael J. Pelczar, 1986. Dasar-Dasar Mikrobiologi. Universitas Indonesia Press.Jakarta
Monack D.M., D.M. Bouley and S. Falkow. 2004. Salmonella typhimurium Persists within Macrophages in the Mesenteric Lymph Nodes of Chronically Infected Nramp1+/+ Mice and Can Be Reactivated by IFN Neutralization. J. Exp. Med. Volume 199, Number 2, January 19 , 2004. 231–241. The Rockefeller University Press.
Nuha, U. 2013. Identifikasi dan Karakterisasi Escherichia coli Pada Jus Buah yang Dijual Di Sekitar Kampus Universitas Jember dan Pemanfaatannya Sebagai Sumber Suplemen. Skripsi. Jember
Nursal, S. Wulandari dan S.W. Juwita, 2006. Bioaktifitas Ekstrak Jahe (Zingiber officinale Roxb.) dalam Menghambat Pertumbuhan Koloni Bakteri Escherichia coli dan Bacillus subtilis. Jurnal Biogenesis Vol. 2(2):64-66, 2006.
Pelczar, Jr. M.J. and R.D. Reid. 1965. Microbiology. McGraw Hill Book, New York.
Permata F.S., 2009. Diagnosis Salmonellosis Disertai Scabies Kelinci E
http://koas.vet-klinik.com/index.php/diagnosis-salmonellosis- disertai-scabies-kelinci. Diakses pada tanggal 03 January 2009.
Suprapto, D., 2008. Salmonella Bahaya Tak
Terlihat, http://www.kharisma.de/?q=node/176, diakses pada tanggal 11 Maret 2008.
Susan M.N., M. Poeloengan dan T. Yulianti, 2006. Analisis Senyawa Kimia Sekunder dan Uji Daya Antibakteri Ekstrak Daun Tanjung (Mimusops elengi L) Terhadap Salmonella typhi dan Shigella boydii. Disampaikan dalam Seminar Nasional Teknologi peternakan dan Vateriner, 2006. Bogor.
Sutedjo, Mul Mulyani. 1996. Mikrobiologi Tanah. Rineka Cipta : Jakarta
Volk, 1993. Microbiology: an introduction. (edisi ke-8th ed,). Benjamin Cummings.Francisco
https://webcache.googleusercontent.com/search?q=cache:DesFJBwSoAs J:https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Hardy CHROMSSAgar.html+&cd=3&hl=id&ct=clnk&gl=id
https://www.scribd.com/doc/218981271/Escherichia-Coli
http://contohdokumen.com/proses-identifikasi-escherichia-coli-pada-sayur- urab-laporan-praktikum-mikrobiologi/
http://www.iccri.net/pengolahan-kakao/
Christina Oktora Matondang, Balai Besar Perbenihan Dan Proteksi Tanaman Perkebunan Medan
<strong>International Review of Practical Innovation, Technology And Green Energy (IRPITAGE)</strong> is a scientific journal that presents the results of scientific works sourced from Community Service in Indonesia. Contains All Forms of Novelty Innovations in both scientific science and technology, as well as issues of limited energy and the social environment in society.This journal is intended as a medium for scientific studies of research results from implementation to the community, thoughts and critical-analytic studies on various issues that can be utilized both nationally and internationally. The scientific article is in the form of a study of the implementation of Community Service that can be accounted for and disseminated nationally and internationally. IRPITAGE Journal from Radja Publika as part of the spirit of disseminating knowledge resulting from community service carried out by researchers in Indonesia. The IRPITAGE Journal from Radja Publika provides articles that can be downloaded for free. With a schedule of publications 3 (three) times a year.